Construction of plants resistant to TYLCV by using artificial zinc-finger proteins

Inhibition of virus DNA replication by artificial zinc finger proteins.

Prevention of virus infections is a major objective in agriculture and human health. One attractive approach to the prevention is inhibition of virus replication. To demonstrate this concept in vivo, an artificial zinc finger protein (AZP) targeting the replication origin of the Beet severe curly top virus (BSCTV), a model DNA virus, was created. In vitro DNA binding assays indicated that the A...

Natural and artificial zinc finger proteins

Zinc finger proteins acquire DNA-binding ability by Zn (II) complexation. In the zinc finger domain of the Cys2His2 type, each finger is approximately 30 amino acid residues long and consists of a simple ββα–fold stabilized by chelation of a zinc ion with the conserved Cys2His2 residues. A zinc finger motif of Cys2His2 offers an attractive framework for the design of a novel DNA-binding protein...

Three Subfamilies of KRAB Zinc Finger Proteins

The Ikaros family of zinc-finger proteins

Ikaros represents a zinc-finger protein family important for lymphocyte development and certain other physiological processes. The number of family members is large, with alternative splicing producing various additional isoforms from each of the five homologous genes in the family. The functional forms of Ikaros proteins could be even more diverse due to protein-protein interactions readily es...

A Scalable Cellular Logic Technology Using Zinc-Finger Proteins

Simple cellular logic circuits have been built by engineering the DNA of host cells. Similar to systems found in nature, these circuits use repressor protein concentrations as logic signals; a gate’s input repressors interact with the cell’s DNA to influence the production of the gate’s output repressors. A limitation in building these circuits is the number of unique repressor proteins availab...